This calculator performs a mathematical calculation based solely on values entered by the user. It is provided as a convenience tool and does not validate, verify, approve, recommend, or document testing activities.

Gain determination

Optimum Gain Calculator

Calculates a gain value from the entered signal and reader parameters. Results update automatically as you type.

1

Enter the maximum dRFU value stated by your instrument (e.g. Nebula 60,000; Agilent 100,000).

dRFU
2Regression type

Sets the target optimum dRFU: 5% of the maximum (linear) or 0.75% (non-linear).

Target optimum dRFU: 5,000 dRFU

3

Gain used for the gain determination measurement.

4Measured dRFU at 0.5 EU/mL PPC

Enter the individual replicate readings — the mean is calculated automatically and used in the formula.

Mean dRFU —

Result

Calculated gain
—
  • Current gain—
  • Optimum dRFU—
  • Mean dRFU—
  • Replicates (n)—
  • Correction factor—
Optimum Gain Calculator — printout
Calculation aid only; no records are stored, exported, approved, or maintained by this tool.

Formula

Correlation between gain and signal development (dRFU).

Calculation steps

  • 1
    Optimum dRFU = maximum dRFU × regression factor
    —
  • 2
    Ratio = optimum dRFU ÷ measured dRFU
    —
  • 3
    log₁₀(ratio)
    —
  • 4
    Exponent = log₁₀(ratio) ÷ 7.2
    —
  • 5
    Correction factor = 10 ^ exponent
    —
  • 6
    Calculated gain (exact) = current gain × correction factor
    —
  • 7
    Rounded to nearest integer
    —
Current gain
Gain used for the gain determination measurement.
Optimum dRFU
Linear regression: 5% of the reader's maximum dRFU. Non-linear regression: 0.75% of the maximum dRFU. (e.g. 5,000 of 100,000)
Current dRFU
Mean dRFU of the 0.5 EU/mL CSE/PPC across all replicates.